Unknown

Dataset Information

0

Expression of AA4.1 marks lymphohematopoietic progenitors in early mouse development.


ABSTRACT: The hematopoietic system of mice is established during the early to midgestational stage of development. However, the earliest lymphohematopoietic progenitors that appear during mouse development have been less well characterized compared with the hematopoietic stem cell compartment of fetal liver and bone marrow. We isolated the earliest lymphohematopoietic progenitors by using embryonic stem (ES) cell culture in vitro. Cells with the c-Kit(+)Lin(-) cell surface phenotype were present abundantly in ES cells cocultured with stromal cell lines. We further separated the cells into two distinct cell subsets based on AA4.1 expression. Although AA4.1(+) and AA4.1(-) cells had equivalent potency to generate myeloid cell lineages, the lymphoid potential in ES-cell-derived cells was largely restricted to the cells expressing AA4.1. The same cell type was present abundantly in the early yolk sac and in fewer numbers (approximately 5% of that in the yolk sac) in the caudal half of the developing embryos. These data suggest that AA4.1 is a cell surface marker that can identify the earliest lymphohematopoietic progenitors in mouse development.

SUBMITTER: Yamane T 

PROVIDER: S-EPMC2690003 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Expression of AA4.1 marks lymphohematopoietic progenitors in early mouse development.

Yamane Toshiyuki T   Hosen Naoki N   Yamazaki Hidetoshi H   Weissman Irving L IL  

Proceedings of the National Academy of Sciences of the United States of America 20090519 22


The hematopoietic system of mice is established during the early to midgestational stage of development. However, the earliest lymphohematopoietic progenitors that appear during mouse development have been less well characterized compared with the hematopoietic stem cell compartment of fetal liver and bone marrow. We isolated the earliest lymphohematopoietic progenitors by using embryonic stem (ES) cell culture in vitro. Cells with the c-Kit(+)Lin(-) cell surface phenotype were present abundantl  ...[more]

Similar Datasets

| S-EPMC3907951 | biostudies-literature
| S-EPMC3871389 | biostudies-literature
| S-EPMC8261706 | biostudies-literature
| S-EPMC4051418 | biostudies-literature
| S-EPMC9198054 | biostudies-literature
| S-EPMC4842327 | biostudies-literature
| S-EPMC5748853 | biostudies-literature
| S-EPMC8313239 | biostudies-literature
| S-EPMC3669380 | biostudies-literature
| S-EPMC3188537 | biostudies-literature