Inhibition of adrenocortical carcinoma cell proliferation by steroidogenic factor-1 inverse agonists.

ABSTRACT:

Context

Transcription factor steroidogenic factor-1 (SF-1) plays a pivotal role in the control of adrenocortical cell steroidogenesis and proliferation. SF-1 amplification and overexpression are found in most cases of childhood adrenocortical tumors (ACTs).

Objective

Our objective was to investigate the effect of SF-1 inverse agonists of the alkyloxyphenol and isoquinolinone classes on the proliferation of human adrenocortical cell lines expressing SF-1 (H295R), in conditions of basal and increased SF-1 expression, or negative for SF-1 expression (SW-13).

Main outcome measures

Proliferation assays, immunoblots, flow cytometric analyses, steroid hormone assays, and reverse transcription quantitative PCR were used.

Results

SF-1 inhibitors of the alkyloxyphenol cl

SUBMITTER: Doghman M 

PROVIDER: S-EPMC2690427 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature