Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry.

ABSTRACT: The spread of cancer cells from a primary tumor to form metastasis at distant sites is a complex multistep process. The cancer cell proteins and plasma membrane proteins in particular involved in this process are poorly defined, and a study of the very early events of the metastatic process using clinical samples or in vitro assays is not feasible. We have used a unique model system consisting of two isogenic human breast cancer cell lines that are equally tumorigenic in mice; but although one gives rise to metastasis, the other disseminates single cells that remain dormant at distant organs. Membrane purification and comparative quantitative LC-MS/MS proteomics identified 13 membrane proteins that were expressed at higher levels and three that were underexpressed in the metastatic compare

SUBMITTER: Leth-Larsen R 

PROVIDER: S-EPMC2690497 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature