Placenta-derived fetal specific mRNA is more readily detectable in maternal plasma than in whole blood.

ABSTRACT:

Background

Placental mRNA was detected in maternal whole blood, raising the possibility of using maternal blood for noninvasive prenatal diagnosis. We investigated fetal mRNA detection in maternal whole blood and determined if it offered advantages over maternal plasma analysis.

Methodology

The concentrations of placental expressed genes, CSH1, KISS1, PLAC4 and PLAC1 in plasma and whole blood from healthy pregnant and non-pregnant individuals were compared by real-time quantitative reverse-transcriptase polymerase chain reaction analysis. Their fetal specificity was investigated by comparing the transcript concentrations in pre- and post-delivery samples and through SNP genotyping by matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry. The gene e

SUBMITTER: Heung MM 

PROVIDER: S-EPMC2690655 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature