Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor.

ABSTRACT:

Aim

To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated.

Methods

A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer.

Results

Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were

SUBMITTER: Wang XY 

PROVIDER: S-EPMC2690667 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature