Aging is not associated with proteasome impairment in UPS reporter mice.

ABSTRACT:

Background

Covalent linkage of ubiquitin regulates the function and, ultimately, the degradation of many proteins by the ubiquitin-proteasome system (UPS). Given its essential role in protein regulation, even slight perturbations in UPS activity can substantially impair cellular function.

Methodology/principal findings

We have generated and characterized a novel transgenic mouse model which expresses a previously described reporter for UPS function. This UPS reporter contains a degron sequence attached to the C-terminus of green fluorescent protein, and is predominantly expressed in neurons throughout the brain of our transgenic model. We then demonstrated that this reporter system is sensitive to UPS inhibition in vivo.

Conclusions/significance

Given the obstacles as

SUBMITTER: Cook C 

PROVIDER: S-EPMC2690827 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature