Directed mammalian gene regulatory networks using expression and comparative genomic hybridization microarray data from radiation hybrids.

ABSTRACT: Meiotic mapping of quantitative trait loci regulating expression (eQTLs) has allowed the construction of gene networks. However, the limited mapping resolution of these studies has meant that genotype data are largely ignored, leading to undirected networks that fail to capture regulatory hierarchies. Here we use high resolution mapping of copy number eQTLs (ceQTLs) in a mouse-hamster radiation hybrid (RH) panel to construct directed genetic networks in the mammalian cell. The RH network covering 20,145 mouse genes had significant overlap with, and similar topological structures to, existing biological networks. Upregulated edges in the RH network had significantly more overlap than downregulated. This suggests repressive relationships between genes are missed by existing approaches, perha

SUBMITTER: Ahn S 

PROVIDER: S-EPMC2690838 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature