Increased isolevuglandin-modified proteins in glaucomatous astrocytes.

ABSTRACT:

Purpose

Lipid oxidation has been proposed to be a factor in the pathophysiology of glaucoma. We investigated whether elevated levels of isolevuglandin (iso[4]LGE(2)) protein adducts are associated with astrocytes derived from the glaucomatous optic nerve head. In addition, we examined whether the iso[4]LGE(2) protein adducts are altered following exposure of astrocytes to elevated pressure.

Methods

Astrocytes were isolated from rat brain cortex and human optic nerve and were subjected to pressure treatments, western blot analyses, liquid chromatography tandem mass spectrometry, and immunocytochemistry.

Results

Elevated levels of isolevuglandin (iso[4]LGE(2)) protein adducts were associated with astrocytes derived from the glaucomatous (n=10) optic nerve head when comp

SUBMITTER: Govindarajan B 

PROVIDER: S-EPMC2690965 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature