Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.

ABSTRACT:

Background

In recent years RNAi screening has proven a powerful tool for dissecting gene functions in animal cells in culture. However, to date, most RNAi screens have been performed in a single cell line, and results then extrapolated across cell types and systems.

Results

Here, to dissect generic and cell type-specific mechanisms underlying cell morphology, we have performed identical kinome RNAi screens in six different Drosophila cell lines, derived from two distinct tissues of origin. This analysis identified a core set of kinases required for normal cell morphology in all lines tested, together with a number of kinases with cell type-specific functions. Most significantly, the screen identified a role for minibrain (mnb/DYRK1A), a kinase associated with Down's syndrome

SUBMITTER: Liu T 

PROVIDER: S-EPMC2690997 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature