New real-time PCR-based method for Kingella kingae DNA detection: application to samples collected from 89 children with acute arthritis.

ABSTRACT: Inoculation of blood culture vials with joint fluid samples has revealed the important pathogenic role of Kingella kingae in pediatric arthritis. However, recent studies based on broad-range 16S ribosomal DNA PCR and real-time PCR without a probe suggest that conventional methods remain suboptimal. We developed a new real-time PCR method with a probe that is highly specific for K. kingae and applied it to joint fluid samples collected from 89 children with suspected arthritis admitted to our institution during a 2-year period. Real-time PCR was also applied to blood samples obtained before surgery and to joint drainage fluid samples obtained during several days after surgery. Thirty-six (40%) of the 89 cases of suspected septic arthritis had positive culture. Staphylococcus aureus was the

SUBMITTER: Ilharreborde B 

PROVIDER: S-EPMC2691089 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature