Elevated polyamines induce c-MYC overexpression by perturbing quadruplex-WC duplex equilibrium.

ABSTRACT: The biological role of quadruplexes and polyamines has been independently associated with cancer. However, quadruplex-polyamine mediated transcriptional regulation remain unaddressed. Herein, using c-MYC quadruplex model, we have attempted to understand quadruplex-polyamine interaction and its role in transcriptional regulation. We initially employed biophysical approach involving CD, UV and FRET to understand the role of polyamines (spermidine and spermine) on conformation, stability, molecular recognition of quadruplex and to investigate the effect of polyamines on quadruplex-Watson Crick duplex transition. Our study demonstrates that polyamines affect the c-MYC quadruplex conformation, perturb its recognition properties and delays duplex formation. The relative free energy difference (D

SUBMITTER: Kumar N 

PROVIDER: S-EPMC2691834 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature