A hierarchical Bayesian model for comparing transcriptomes at the individual transcript isoform level.

ABSTRACT: The complexity of mammalian transcriptomes is compounded by alternative splicing which allows one gene to produce multiple transcript isoforms. However, transcriptome comparison has been limited to differential analysis at the gene level instead of the individual transcript isoform level. High-throughput sequencing technologies and high-resolution tiling arrays provide an unprecedented opportunity to compare transcriptomes at the level of individual splice variants. However, sequence read coverage or probe intensity at each position may represent a family of splice variants instead of one single isoform. Here we propose a hierarchical Bayesian model, BASIS (Bayesian Analysis of Splicing IsoformS), to infer the differential expression level of each transcript isoform in response to two cond

SUBMITTER: Zheng S 

PROVIDER: S-EPMC2691848 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature