Delineating bacteriostatic and bactericidal targets in mycobacteria using IPTG inducible antisense expression.

ABSTRACT: In order to identify novel high value antibacterial targets it is desirable to delineate whether the inactivation of the target enzyme will lead to bacterial death or stasis. This knowledge is particularly important in slow growing organisms, like mycobacteria, where most of the viable anti-tubercular agents are bactericidal. A bactericidal target can be identified through the conditional deletion or inactivation of the target gene at a relatively high cell number and subsequently following the time course of survival for the bacteria. A simple protocol to execute conditional inactivation of a gene is by antisense expression. We have developed a mycobacteria specific IPTG inducible vector system and monitored the effect of antisense inhibition of several known essential genes in mycobacter

SUBMITTER: Kaur P 

PROVIDER: S-EPMC2691988 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature