Identification of transformation-related pathways in a breast epithelial cell model using a ribonomics approach.

ABSTRACT: The aberrant expression of many genes is a common feature in the malignant transformation of cells. In mammalian cells, posttranscriptional gene regulatory processes are emerging as critical determinants controlling gene expression both in physiologic and pathologic conditions. These regulatory mechanisms are directed primarily by the interaction of mRNAs with specific RNA-binding proteins (RBP). There is an emerging body of data demonstrating that two RBPs, AUF1 and HuR, can antagonistically affect the posttranscriptional fate of target mRNAs, as well as concurrently bind to common target transcripts. Employing MCT-1 oncogene-mediated transformation of immortalized breast epithelial MCF10A cells, we characterized the largely reciprocal association of these two RBPs with target mRNAs and t

SUBMITTER: Mazan-Mamczarz K 

PROVIDER: S-EPMC2692251 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature