Mechanism and dynamics of translesion DNA synthesis catalyzed by the Escherichia coli Klenow fragment.

ABSTRACT: Translesion DNA synthesis represents the ability of a DNA polymerase to incorporate and extend beyond damaged DNA. In this report, the mechanism and dynamics by which the Escherichia coli Klenow fragment performs translesion DNA synthesis during the misreplication of an abasic site were investigated using a series of natural and non-natural nucleotides. Like most other high-fidelity DNA polymerases, the Klenow fragment follows the "A-rule" of translesion DNA synthesis by preferentially incorporating dATP opposite the noninstructional lesion. However, several 5-substituted indolyl nucleotides lacking classical hydrogen-bonding groups are incorporated approximately 100-fold more efficiently than the natural nucleotide. In general, analogues that contain large substituent groups in conjunctio

SUBMITTER: Sheriff A 

PROVIDER: S-EPMC2692461 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature