Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage.

ABSTRACT:

Objective

An age-related decline in chondrocyte production of osteogenic protein-1 (OP-1) (Bone Morphogenetic Protein-7) may contribute to cartilage loss in osteoarthritis. This study was designed to determine if increased methylation of the OP-1 promoter might serve as a mechanism for the age-related decline in OP-1 expression.

Methods

Human articular chondrocytes were isolated from cartilage obtained after death from tissue donors (ages 19-86 years) without a known history of arthritis. DNA was obtained from isolated chondrocytes in primary culture and analyzed for OP-1 promoter methylation by polymerase chain reaction (PCR) after bisulfite treatment. Cultured cells were treated with the DNA methyltransferase inhibitor 5-azacytidine and OP-1 production was measured in the

SUBMITTER: Loeser RF 

PROVIDER: S-EPMC2692619 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature