Cigarette smoke activates NF kappa B and induces Muc5b expression in mouse middle ear cells.

ABSTRACT:

Objectives

Cigarette smoke exposure is a significant risk factor in the development of otitis media (OM). Nuclear factor kappa B (NF-kappa B) is a ubiquitous transcription factor known to mediate cigarette smoke effects on gene regulation in multiple cell types. The MUC5B mucin gene contains several putative NF-kappa B sites in its promoter and is the predominant mucin expressed in human OM. We hypothesized that in vitro stimulation of a recently developed model system, murine middle ear epithelial cells (MEEC), with cigarette smoke condensate (CSC) activates NF-kappa B and subsequently induces Muc5b gene expression.

Methods

Luciferase reporter assays, electromobility shift assays (EMSA), and quantitative microplate transcription factor assays (TFA) were performed to evaluat

SUBMITTER: Preciado D 

PROVIDER: S-EPMC2692718 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature