Glycogene expression in conjunctiva of patients with dry eye: downregulation of Notch signaling.

ABSTRACT:

Purpose

Glycoconjugates regulate a variety of biological events in mucosal surfaces, such as differentiation of postmitotic epithelial cells and maintenance of the wet-surfaced phenotype. This study aimed to identify the repertoire of genes (glycogenes) involved in biosynthesis of glycoconjugates in conjunctiva of normal subjects and patients with dry eye.

Methods

RNA from conjunctival impression cytology samples was amplified and hybridized to a custom-designed glycogene microarray. Intensity data were converted to expression values and analyzed by ANOVA. Microarray data for selected Notch glycogenes were confirmed by quantitative real-time PCR. Notch receptors and ligands were immunolocalized on conjunctival biopsies by confocal microscopy.

Results

By microarray, 42

SUBMITTER: Mantelli F 

PROVIDER: S-EPMC2693254 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature