Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

ABSTRACT: Human ductal saliva contributes over a thousand unique proteins to whole oral fluids. The mechanism by which most of these proteins are secreted by salivary glands remains to be determined. The present study used a mass spectrometry-based, shotgun proteomics approach to explore the possibility that a subset of the proteins found in saliva are derived from exosomes, membrane-bound vesicles of endosomal origin within multivesicular endosomes. Using MudPIT (multidimensional protein identification technology) mass spectrometry, we catalogued 491 proteins in the exosome fraction of human parotid saliva. Many of these proteins were previously observed in ductal saliva from parotid glands (265 proteins). Furthermore, 72 of the proteins in parotid exosomes overlap with those previously identified

SUBMITTER: Gonzalez-Begne M 

PROVIDER: S-EPMC2693447 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature