COOH-terminal deletion of HBx gene is a frequent event in HBV-associated hepatocellular carcinoma.

ABSTRACT:

Aim

To investigate the hepatitis B virus (HBV)x gene (HBx) state in the tissues of HBV-related hepatocellular carcinoma (HCC) in Chinese patients and whether there were particular HBx mutations.

Methods

HBx gene was amplified and direct sequencing was used in genomic DNA samples from 20 HCC and corresponding non-cancerous liver tissues from HBsAg-positive patients. HBV DNA integration and HBx deleted mutation were validated in 45 HCC patients at different stages by Southern blot analysis and polymerase chain reaction methods.

Results

The frequencies of HBx point mutations were significantly lower in HCC than their corresponding non-cancerous liver tissues (11/19 vs 18/19, P=0.019). In contrast, deletions in HBx gene were significantly higher in HCC than their non-canc

SUBMITTER: Liu XH 

PROVIDER: S-EPMC2693680 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature