Large-scale analysis of antisense transcription in wheat using the Affymetrix GeneChip Wheat Genome Array.

ABSTRACT:

Background

Natural antisense transcripts (NATs) are transcripts of the opposite DNA strand to the sense-strand either at the same locus (cis-encoded) or a different locus (trans-encoded). They can affect gene expression at multiple stages including transcription, RNA processing and transport, and translation. NATs give rise to sense-antisense transcript pairs and the number of these identified has escalated greatly with the availability of DNA sequencing resources and public databases. Traditionally, NATs were identified by the alignment of full-length cDNAs or expressed sequence tags to genome sequences, but an alternative method for large-scale detection of sense-antisense transcript pairs involves the use of microarrays. In this study we developed a novel protocol to assay sense

SUBMITTER: Coram TE 

PROVIDER: S-EPMC2694213 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature