Preclinical evaluation of a replication-deficient intranasal DeltaNS1 H5N1 influenza vaccine.

ABSTRACT:

Background

We developed a novel intranasal influenza vaccine approach that is based on the construction of replication-deficient vaccine viruses that lack the entire NS1 gene (DeltaNS1 virus). We previously showed that these viruses undergo abortive replication in the respiratory tract of animals. The local release of type I interferons and other cytokines and chemokines in the upper respiratory tract may have a "self-adjuvant effect", in turn increasing vaccine immunogenicity. As a result, DeltaNS1 viruses elicit strong B- and T- cell mediated immune responses.

Methodology/principal findings

We applied this technology to the development of a pandemic H5N1 vaccine candidate. The vaccine virus was constructed by reverse genetics in Vero cells, as a 5:3 reassortant, encoding f

SUBMITTER: Romanova J 

PROVIDER: S-EPMC2694350 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature