Microarray analysis of RNA extracted from formalin-fixed, paraffin-embedded and matched fresh-frozen ovarian adenocarcinomas.

ABSTRACT:

Background

Gene expression profiling of formalin-fixed, paraffin-embedded (FFPE) samples represents a valuable approach for advancing oncology diagnostics and enhancing retrospective clinical studies; however, at present, this methodology still requires optimization and thus has not been extensively used. Here, we utilized thorough quality control methods to assess RNA extracted from FFPE samples and then compared it to RNA extracted from matched fresh-frozen (FF) counterparts. We preformed genome-wide expression profiling of FF and FFPE ovarian serous adenocarcinoma sample pairs and compared their gene signatures to normal ovary samples.

Methods

RNA from FFPE samples was extracted using two different methods, Ambion and Agencourt, and its quality was determined by profiling

SUBMITTER: Fedorowicz G 

PROVIDER: S-EPMC2694827 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature