Biases in Drosophila melanogaster protein trap screens.

ABSTRACT:

Background

The ability to localise or follow endogenous proteins in real time in vivo is of tremendous utility for cell biology or systems biology studies. Protein trap screens utilise the random genomic insertion of a transposon-borne artificial reporter exon (e.g. encoding the green fluorescent protein, GFP) into an intron of an endogenous gene to generate a fluorescent fusion protein. Despite recent efforts aimed at achieving comprehensive coverage of the genes encoded in the Drosophila genome, the repertoire of genes that yield protein traps is still small.

Results

We analysed the collection of available protein trap lines in Drosophila melanogaster and identified potential biases that are likely to restrict genome coverage in protein trap screens. The protein trap scree

SUBMITTER: Aleksic J 

PROVIDER: S-EPMC2695487 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature