A model for the spatiotemporal organization of DNA replication in Saccharomyces cerevisiae.

ABSTRACT: DNA replication in eukaryotes is considered to proceed according to a precise program in which each chromosomal region is duplicated in a defined temporal order. However, recent studies reveal an intrinsic temporal disorder in the replication of yeast chromosome VI. Here we provide a model of the chromosomal duplication to study the temporal sequence of origin activation in budding yeast. The model comprises four parameters that influence the DNA replication system: the lengths of the chromosomes, the explicit chromosomal positions for all replication origins as well as their distinct initiation times and the replication fork migration rate. The designed model is able to reproduce the available experimental data in form of replication profiles. The dynamics of DNA replication was monitored

SUBMITTER: Spiesser TW 

PROVIDER: S-EPMC2695552 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature