Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos.

ABSTRACT:

Background

Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning.

Results

Bovine clones from up to four generations of successive cloning were produced by chromatin transfer (CT). Using Affymetrix bovine microarrays we determined that the transcriptomes of blastocysts derived from the first and the fourth rounds of cloning (CT1 and CT4 respectively) have undergone an extensive r

SUBMITTER: Rodriguez-Osorio N 

PROVIDER: S-EPMC2695822 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature