Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis.

ABSTRACT: Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.

SUBMITTER: Khrapunov S 

PROVIDER: S-EPMC2696129 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature