Influence of fatty acid diets on gene expression in rat mammary epithelial cells.

ABSTRACT:

Background

This study examines the impact of dietary fatty acids on regulation of gene expression in mammary epithelial cells before and during puberty.

Methods

Diets primarily consisted of n-9 monounsaturated fatty acids (olive oil), n-6 polyunsaturated fatty acids (safflower), saturated acids (butter), and the reference AIN-93G diet (soy oil). The dietary regimen mimics the repetitive nature of fatty acid exposure in Western diets. Diet-induced changes in gene expression were examined in laser capture microdissected mammary ductal epithelial cells at day of weaning and end of puberty. PCNA immunohistochemistry analysis compared proliferation rates between diets.

Results

Genes differentially expressed between each test diets and the reference diet were significantly

SUBMITTER: Medvedovic M 

PROVIDER: S-EPMC2696152 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature