An improved method for genome wide DNA methylation profiling correlated to transcription and genomic instability in two breast cancer cell lines.

ABSTRACT:

Background

DNA methylation is a widely studied epigenetic mechanism known to correlate with gene repression and genomic stability. Development of sensitive methods for global detection of DNA methylation events is of particular importance.

Results

We here describe a technique, called modified methylation-specific digital karyotyping (MMSDK) based on methylation-specific digital karyotyping (MSDK) with a novel sequencing approach. Briefly, after a tandem digestion of genomic DNA with a methylation-sensitive mapping enzyme and a fragmenting enzyme, short sequence tags are obtained. These tags are amplified, followed by direct, massively parallel sequencing (Solexa 1G Genome Analyzer). This method allows high-throughput and low-cost genome-wide DNA methylation mapping. We appli

SUBMITTER: Li J 

PROVIDER: S-EPMC2696471 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature