Optimization of oligonucleotide microarray fabricated by spotting 65-mer.

ABSTRACT: DNA microarrays currently provide measurements of sufficiently high quality to allow a wide variety of sound inferences about gene regulation and the coordination of cellular processes to be drawn. Nonetheless, a desire for greater precision in the measurements continues to drive the microarray research community to seek higher measurement quality through improvements in array fabrication and sample labeling and hybridization. We prepared oligonucleotide microarrays by printing 65-mer on aldehyde functional group-derivatized slides as described in a previous study. We could improve the reliability of data by removing enzymatic bias during probe labeling and hybridizing under a more stringent condition. This optimized method was used to profile gene expression patterns for nine different mo

SUBMITTER: Lee M 

PROVIDER: S-EPMC2697255 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature