Marked changes in signal transduction upon heteromerization of dopamine D1 and histamine H3 receptors.

ABSTRACT:

Background and purpose

Functional interactions between the G protein-coupled dopamine D1 and histamine H3 receptors have been described in the brain. In the present study we investigated the existence of D1-H3 receptor heteromers and their biochemical characteristics.

Experimental approach

D1-H3 receptor heteromerization was studied in mammalian transfected cells with Bioluminescence Resonance Energy Transfer and binding assays. Furthermore, signalling through mitogen-activated protein kinase (MAPK) and adenylyl cyclase pathways was studied in co-transfected cells and compared with cells transfected with either D1 or H3 receptors.

Key results

Bioluminescence Resonance Energy Transfer and binding assays confirmed that D1 and H3 receptors can heteromerize. Activation of

SUBMITTER: Ferrada C 

PROVIDER: S-EPMC2697789 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature