Using morpholinos for gene knockdown in Giardia intestinalis.

ABSTRACT: We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

SUBMITTER: Carpenter ML 

PROVIDER: S-EPMC2698301 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature