Dataset Information

Using morpholinos for gene knockdown in Giardia intestinalis.

ABSTRACT: We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

SUBMITTER: Carpenter ML

PROVIDER: S-EPMC2698301 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Using morpholinos for gene knockdown in Giardia intestinalis.

Carpenter Meredith L ML Cande W Zacheus WZ

Eukaryotic cell 20090417 6

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies. ...[more]

PMID: 19377039

Dataset Information

Using morpholinos for gene knockdown in Giardia intestinalis.

Publications

Using morpholinos for gene knockdown in Giardia intestinalis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Similar Datasets

Transcriptome profiling of Giardia intestinalis using strand-specific RNA-seq.
| S-EPMC3610916 | biostudies-literature

Coordinated Changes in Gene Expression Throughout Encystation of Giardia intestinalis.
| S-EPMC4807828 | biostudies-literature

Differential gene expression during encystation in Giardia intestinalis
2022-01-10 | GSE141795 | GEO