Phenol degradation in the strictly anaerobic iron-reducing bacterium Geobacter metallireducens GS-15.

ABSTRACT: Information on anaerobic phenol metabolism by physiological groups of bacteria other than nitrate reducers is scarce. We investigated phenol degradation in the strictly anaerobic iron-reducing deltaproteobacterium Geobacter metallireducens GS-15 using metabolite, transcriptome, proteome, and enzyme analyses. The results showed that the initial steps of phenol degradation are accomplished by phenylphosphate synthase (encoded by pps genes) and phenylphosphate carboxylase (encoded by ppc genes) as known from Thauera aromatica, but they also revealed some distinct differences. The pps-ppc gene cluster identified in the genome is functional, as shown by transcription analysis. In contrast to T. aromatica, transcription of the pps- and ppc-like genes was induced not only during growth on phenol,

SUBMITTER: Schleinitz KM 

PROVIDER: S-EPMC2698347 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature