Transcriptome and secretome analyses of Phanerochaete chrysosporium reveal complex patterns of gene expression.

ABSTRACT: The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of significant changes in transcript accumulation and/or peptide sequences of the secreted proteins. Under nitrogen or carbon limitation, lignin and manganese peroxidase expression increased relative to nutrient replete medium. Various extracellular oxidases were also secreted in these media, supporting a physiological connection based on peroxide generation. Numerous genes presumed to be involved in mobilizing and recycling nitrogen were expressed under nitrogen limitation, and among th

SUBMITTER: Vanden Wymelenberg A 

PROVIDER: S-EPMC2698378 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature