Deconvolution of blood microarray data identifies cellular activation patterns in systemic lupus erythematosus.

ABSTRACT: Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease with a complex spectrum of cellular and molecular characteristics including several dramatic changes in the populations of peripheral leukocytes. These changes include general leukopenia, activation of B and T cells, and maturation of granulocytes. The manifestation of SLE in peripheral blood is central to the disease but is incompletely understood. A technique for rigorously characterizing changes in mixed populations of cells, microarray expression deconvolution, has been applied to several areas of biology but not to SLE or to blood. Here we demonstrate that microarray expression deconvolution accurately quantifies the constituents of real blood samples and mixtures of immune-derived cell lines. We characterize a broad

SUBMITTER: Abbas AR 

PROVIDER: S-EPMC2699551 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature