Microalbuminuria in type 1 diabetes is associated with enhanced excretion of the endocytic multiligand receptors megalin and cubilin.

ABSTRACT:

Objective

Proteinuria is the hallmark of diabetic nephropathy; yet, glomerular histology does not fully explain mechanisms contributing to proteinuria. Our objective was to identify proteins in the urine of individuals with type 1 diabetes and microalbuminuria that might implicate a mechanistic pathway operative in proteinuria.

Research design and methods

Using a GeLC/MS platform proteomics approach, we compared the urine proteome from 12 healthy nondiabetic individuals, 12 subjects with type 1 diabetes yet normal urinary albumin excretion rates, and 12 subjects with type 1 diabetes and microalbuminuria (type 1 diabetes + microalbuminuria).

Results

The abundance of megalin and cubilin, two multiligand receptors expressed in kidney proximal tubule cells and involved wi

SUBMITTER: Thrailkill KM 

PROVIDER: S-EPMC2699744 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature