Gene expression during Drosophila melanogaster egg development before and after reproductive diapause.

ABSTRACT:

Background

Despite the importance of egg development to the female life cycle in Drosophila, global patterns of gene expression have not been examined in detail, primarily due to the difficulty in isolating synchronised developmental stages in sufficient quantities for gene expression profiling. Entry into vitellogenesis is a key stage of oogenesis and by forcing females into reproductive diapause we are able to arrest oogenesis at the pre-vitellogenic stages. Releasing females from diapause allows collection of relatively synchronous developing egg populations and an investigation of some of the transcriptional dynamics apparent before and after reproductive diapause.

Results

Focusing on gender-biased transcription, we identified mechanisms of egg development suppressed dur

SUBMITTER: Baker DA 

PROVIDER: S-EPMC2700134 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature