Transcriptome adaptation of group B Streptococcus to growth in human amniotic fluid.

ABSTRACT:

Background

Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of the adaptation of this pathogen to growth in amniotic fluid. To address this knowledge deficit, we characterized the transcriptome of GBS grown in human amniotic fluid (AF) and compared it with the transcriptome in rich laboratory medium.

Methods

GBS was grown in Todd Hewitt-yeast extract medium and human AF. Bacteria were collected at mid-logarithmic, late-logarithmic and stationary growth phase. We performed global expression microarray analysis using a custom-made Affymetrix GeneC

SUBMITTER: Sitkiewicz I 

PROVIDER: S-EPMC2700258 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature