Dexamethasone mediates protection against acute pancreatitis via upregulation of pancreatitis-associated proteins.

ABSTRACT:

Aim

To examine the influence of dexamethasone on pancreatitis-associated protein (PAP) gene expression using both in vitro and in vivo models of acute pancreatitis and to study how PAP gene expression correlates with severity of pancreatitis.

Methods

In vitro, IL-6 stimulated pancreas acinar AR42J cells were cultured with increasing concentrations of dexamethasone and assayed for PAP expression (RT-PCR). In vivo, pancreatitis was induced in rats by retrograde injection of 40 g/L taurocholate into the pancreatic duct. Animals were pretreated with dexamethasone (2 mg/kg) daily or saline for 4 d. Pancreata and serum were harvested after 24 h and gene expression levels of PAP I, II and III were measured by RT-PCR. Severity of pancreatitis was based on serum amylase, pancreatic w

SUBMITTER: Kandil E 

PROVIDER: S-EPMC2700294 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature