Quantitative real time polymerase chain reaction for measurement of human interleukin-5 receptor alpha spliced isoforms mRNA.

ABSTRACT:

Background

Expression of human Interleukin-5 receptor alpha (hIL-5Ralpha) is controlled by alternative splicing, which generates two different transcripts encoding a membrane-anchored and a soluble form of the receptor, respectively. Although the study of the expression and regulation of hIL-5Ralpha is of crucial importance in the field of immunological processing, methods and techniques until now described lack sufficient sensitivity for detection of small differences in the expression of these isoforms. The aim of this study was to develop a reliable and sensitive real-time quantitative PCR assay to analyse the expression level of each isoform.

Methods

For the quantitative real-time PCR assay, two standard curves specific for each splice variant were constructed. PCR ampli

SUBMITTER: Perez C 

PROVIDER: S-EPMC270039 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature