Chemically defined medium and Caenorhabditis elegans.

ABSTRACT:

Background

C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that animals receive. Liquid cultivation allows automated culturing and experimentation and should be of use in large-scale growth and screening of animals.

Results

We find that CeMM is versatile and culturing is simple. CeMM can be used in a solid or liquid state, it can be stored unused for at least a year, unattended actively growing cultures may be maintained longer than with standard techniques, and standard C. elegans protocols work well with animals grown in defined medium. We also find that there are caveats to using defined medium. Animals in defined

SUBMITTER: Szewczyk NJ 

PROVIDER: S-EPMC270041 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature