Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project.

ABSTRACT:

Background

Subtractive hybridization methods are valuable tools for identifying differentially regulated genes in a given tissue avoiding redundant sequencing of clones representing the same expressed genes, maximizing detection of low abundant transcripts and thus, affecting the efficiency and cost effectiveness of small scale cDNA sequencing projects aimed to the specific identification of useful genes for breeding purposes. The objective of this work is to evaluate alternative strategies to high-throughput sequencing projects for the identification of novel genes differentially expressed in sunflower as a source of organ-specific genetic markers that can be functionally associated to important traits.

Results

Differential organ-specific ESTs were generated from leaf, stem

SUBMITTER: Fernandez P 

PROVIDER: S-EPMC270089 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature