Modulation of gene expression in Actinobacillus pleuropneumoniae exposed to bronchoalveolar fluid.

ABSTRACT:

Background

Actinobacillus pleuropneumoniae, the causative agent of porcine contagious pleuropneumonia, is an important pathogen of swine throughout the world. It must rapidly overcome the innate pulmonary immune defenses of the pig to cause disease. To better understand this process, the objective of this study was to identify genes that are differentially expressed in a medium that mimics the lung environment early in the infection process.

Methods and principal findings

Since bronchoalveolar lavage fluid (BALF) contains innate immune and other components found in the lungs, we examined gene expression of a virulent serovar 1 strain of A. pleuropneumoniae after a 30 min exposure to BALF, using DNA microarrays and real-time PCR. The functional classes of genes found to be up

SUBMITTER: Lone AG 

PROVIDER: S-EPMC2700959 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature