Expression analysis of Barrett's esophagus-associated high-grade dysplasia in laser capture microdissected archival tissue.

ABSTRACT:

Purpose

Identifying genes differentially expressed in nondysplastic BE (NDBE) from those expressed in high-grade dysplasia (HGD) should be of value in improving our understanding of this transition and may yield new diagnostic and/or prognostic markers. The aim of this study was to determine the differential transcriptome of HGD compared with NDBE through gene microarray analysis of epithelial cells microdissected from archival tissue specimens.

Experimental design

Laser capture microdissection was used to isolate epithelial cells from adjacent inflammatory and stromal cells. Epithelial mRNA was extracted from areas of NDBE and HGD in matched biopsies from 11 patients. mRNA was reverse transcribed and applied on Affymetrix cDNA microarray chips customized for formalin-expose

SUBMITTER: Sabo E 

PROVIDER: S-EPMC2701739 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature