Inhibition of chalcone synthase expression in anthers of Raphanus sativus with Ogura male sterile cytoplasm.

ABSTRACT:

Background and aims

Expression of the mitochondrial gene orf138 causes Ogura cytoplasmic male sterility (CMS) in Raphanus sativus, but little is known about the mechanism by which CMS takes place. A preliminary microarray experiment revealed that several nuclear genes concerned with flavonoid biosynthesis were inhibited in the male-sterile phenotype. In particular, a gene for one of the key enzymes for flavonoid biosynthesis, chalcone synthase (CHS), was strongly inhibited. A few reports have suggested that the inhibition of CHS causes nuclear-dependent male sterile expression; however, there do not appear to be any reports elucidating the effect of CHS on CMS expression. In this study, the expression patterns of the early genes in the flavonoid biosynthesis pathway, including CHS,

SUBMITTER: Yang S 

PROVIDER: S-EPMC2701772 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature