Molecular cloning and expression of a novel trehalose synthase gene from Enterobacter hormaechei.

ABSTRACT:

Background

Trehalose synthase (TreS) which converts maltose to trehalose is considered to be a potential biocatalyst for trehalose production. This enzymatic process has the advantage of simple reaction and employs an inexpensive substrate. Therefore, new TreS producing bacteria with suitable enzyme properties are expected to be isolated from extreme environment.

Results

Six TreS producing strains were isolated from a specimen obtained from soil of the Tibetan Plateau using degenerate PCR. A novel treS gene from Enterobacter hormaechei was amplified using thermal asymmetric interlaced PCR. The gene contained a 1626 bp open reading frame encoding 541 amino acids. The gene was expressed in Escherichia coli, and the recombinant TreS was purified and characterized. The purified

SUBMITTER: Yue M 

PROVIDER: S-EPMC2701924 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature