Identification and analysis of miRNAs in human breast cancer and teratoma samples using deep sequencing.

ABSTRACT:

Background

MiRNAs play important roles in cellular control and in various disease states such as cancers, where they may serve as markers or possibly even therapeutics. Identifying the whole repertoire of miRNAs and understanding their expression patterns is therefore an important goal.

Methods

Here we describe the analysis of 454 pyrosequencing of small RNA from four different tissues: Breast cancer, normal adjacent breast, and two teratoma cell lines. We developed a pipeline for identifying new miRNAs, emphasizing extracting and retaining as much data as possible from even noisy sequencing data. We investigated differential expression of miRNAs in the breast cancer and normal adjacent breast samples, and systematically examined the mature sequence end variability of miRNA

SUBMITTER: Nygaard S 

PROVIDER: S-EPMC2702338 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature