Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint.

ABSTRACT:

Background

Plasmids are being reconsidered as viable vector alternatives to viruses for gene therapies and vaccines because they are safer, non-toxic, and simpler to produce. Accordingly, there has been renewed interest in the production of plasmid DNA itself as the therapeutic end-product of a bioprocess. Improvement to the best current yields and productivities of such emerging processes would help ensure economic feasibility on the industrial scale. Our goal, therefore, was to develop a stoichiometric model of Escherichia coli metabolism in order to (1) determine its maximum theoretical plasmid-producing capacity, and to (2) identify factors that significantly impact plasmid production.

Results

Such a model was developed for the production of a high copy plasmid under con

SUBMITTER: Cunningham DS 

PROVIDER: S-EPMC2702362 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature