UROX 2.0: an interactive tool for fitting atomic models into electron-microscopy reconstructions.

ABSTRACT: Electron microscopy of a macromolecular structure can lead to three-dimensional reconstructions with resolutions that are typically in the 30-10 A range and sometimes even beyond 10 A. Fitting atomic models of the individual components of the macromolecular structure (e.g. those obtained by X-ray crystallography or nuclear magnetic resonance) into an electron-microscopy map allows the interpretation of the latter at near-atomic resolution, providing insight into the interactions between the components. Graphical software is presented that was designed for the interactive fitting and refinement of atomic models into electron-microscopy reconstructions. Several characteristics enable it to be applied over a wide range of cases and resolutions. Firstly, calculations are performed in reciproca

SUBMITTER: Siebert X 

PROVIDER: S-EPMC2703571 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature