EST and EST-SSR marker resources for Iris.

ABSTRACT:

Background

Limited DNA sequence and DNA marker resources have been developed for Iris (Iridaceae), a monocot genus of 200-300 species in the Asparagales, several of which are horticulturally important. We mined an I. brevicaulis-I. fulva EST database for simple sequence repeats (SSRs) and developed ortholog-specific EST-SSR markers for genetic mapping and other genotyping applications in Iris. Here, we describe the abundance and other characteristics of SSRs identified in the transcript assembly (EST database) and the cross-species utility and polymorphisms of I. brevicaulis-I. fulva EST-SSR markers among wild collected ecotypes and horticulturally important cultivars.

Results

Collectively, 6,530 ESTs were produced from normalized leaf and root cDNA libraries of I. brevicaul

SUBMITTER: Tang S 

PROVIDER: S-EPMC2703627 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature